Gene Expression Analysis is used to determine whether a gene is expressed and can also determine in which tissues gene expression is turned on. This is important in the initial characterization of genetically engineered mice and rats and can be used as a quality control assay for ongoing monitoring of genetically engineered mice and rats.
Taconic offers the following gene expression services:
Gene Expression Assay Design
Design and validation of a quantitative reverse transcriptase real-time PCR (RT-PCR) assay
Used to assess the mRNA levels of a single gene in a particular tissue
Gene Expression Analysis
Quantitative reverse transcriptase real-time PCR (RT-PCR) assay to assess the mRNA levels of single gene in a particular tissue
Appropriate for the evaluation fo expression of a transgene in tissue(s) of interest
Appropriate for evaluation of expression of endogenous gene in response to a genetic manipulation in tissue(s) of interest
FACS Analysis (Dual-Antibody)
Evaluation of protein expression in blood samples
Appropriate for verification of protein expression on founders and/or breeders for lines that express a transgene in blood
