Gut Microbiome in MetabolismStudies have indicated that germ-free mice have low epididymal fat accumulation and would need 10-30% additional food consumption to maintain the same body mass as mice with normal microbiota1. The gut microbiome plays a significant role in metabolizing complex nutrients and providing key nutrients necessary for host metabolism. A few examples are listed below:
- Gut microbiota contribute up to 10% of daily dietary energy by utilizing unabsorbed starch and converting it into butyrate, propionate, acetate, and pentanoate, making it accessible to host metabolic pathways2. This results in improved glucose tolerance, insulin sensitivity, and enhances gut immunity.
- Gut microbiota-activated G protein-coupled receptor pathways in gut epithelial cells assist in the production of the multifunctional hormone leptin, which plays a role in host physiological functions such as energy metabolism, appetite, and activation of nerve and immune responses3.
- Vitamin K-producing producing gut bacteria, namely Bacteroides fragilis, Eubacterium lentum, Enterobacter agglomerans, Serratia marcescens, and Enterococcus faecium, anaerobically synthesize vitamin K2, which plays an essential role in lowering cholesterol levels and maintaining normal nervous system function. Gut microbiota also serve as an important source of vitamins B5 and B12, and deficiency of these vitamins is linked to insomnia and neuropsychological disorders2.
- Gut microbes, such as Clostridium scindens, play a significant role in shaping the composition of the gut microbiota and protecting the host from an array of infections by producing secondary bile salts4.
Gut Microbiome in Host Immunity and Nervous SystemThe participation of gut microbiota in host immune response and nervous system development, at both local and systemic levels, has been demonstrated. Some key studies are provided below:
- T lymphocytes balance host intestinal homeostasis, possibly enabling gut microbiota to construct their niches in their host without being attacked by host immunity under normal circumstances, hence maintaining intestinal homeostasis3.
- In gnotobiotic mouse models, commensal segmented filamentous bacteria (SFB) were shown to induce intestinal TH17 cells and the production of pro-inflammatory cytokines IL-17 and IL-22, enhancing antimicrobial defense and mucosal immunity against the intestinal pathogen Citrobacter rodentium5.
- The gut microbiome plays a significant role in hematopoiesis (involved in developing innate immunity). Germ-free mice colonized with normal gut microbiota have displayed an increase in the number of microglial cells, also known as macrophages of brain6.
- A decrease in gut microbiota diversity and secondary bile acid production is associated with neuropsychiatric diseases such as depression, Parkinson's disease, and autism3.
Gut Microbiome and DiseasesMicrobiome and Obesity: Study of lean and obese mice demonstrated a contribution of gut microorganisms in utilizing energy from nutrients, including otherwise indigestible dietary polysaccharides, suggesting the role of gut microbiota in host energy balance3. Similarly, studies were performed in humans to characterize the microbial content of fecal microbiomes of twins. The study demonstrated that the bacterial family Christensenellaceae is enriched in lean twins compared to obese twins from the same twin set. To further strengthen the connection between Christensenellaceae and weight, the researchers transplanted fecal microbiota with and without Christensenella consortium, one of the members of Christensenellaceae family, into germ-free mice. A 16S rRNA analysis of the fecal microbiomes before and after transfer showed that C. consortium was present throughout the experiment in recipient mice and remained stable through day 21. Further analysis demonstrated that the percent increase in body weight post-inoculation was lower in mice transplanted with C. consortium in comparison to the control mice without C. consortium7,8.
Figure 1: Crucial role of gut microbiome variability on human health
Gut Microbiome in Colorectal Cancer (CRC): Colorectal cancer arises from a stepwise disturbance of the composition of the gut microbiota, induced by food components or diet, plus genetic alterations in oncogenes and tumor-suppressor genes9. Numerous studies comparing fecal microbiota from CRC patients versus controls have shown that, despite having overall similar microbiomes, sets of significantly enriched and depleted microorganisms can be identified that differentiate CRC and control populations. Bacteria such as Streptococcus gallolyticus, Faecalibacterium nucleatum, Escherichia coli, B. fragilis, and E. faecalis are present in higher amounts in CRC patients as compared to controls, whereas genera such as Roseburia, Clostridium, Faecalibacterium, and Bifidobacterium are generally depleted in CRC patients. Moreover, quantitative analysis of Fusobacterium nucleatum is used as a biomarker to indicate colon cancer disease stage advancement9.
Gut Microbiome in Type 2 Diabetes: In multiple cases, an increased number of bacterial genes have been found in the blood stream and in fecal samples of patients with type 2 diabetes. This indicates the presence of gut dysbiosis (microbial imbalance) and bacterial translocation from gut to blood in patients with the disease. In experiments with mice, high-fat food increased the translocation of live bacteria through intestinal mucosa to blood10. However, further studies are needed to determine the significance of the translocation of Gram-positive bacteria in systemic inflammation identified in obese and type 2 diabetic patients.
Gut Microbiome in Inflammatory Bowel Disease (IBD): Connections between gut microbiota and host factors show a contributing factor in the development of gastrointestinal inflammation and IBD. Reports have indicated elevated levels of pro-inflammatory genes were observed in germ-free mice colonized by gut microbiota isolated from IBD patients. Colitis-prone genetically predisposed germ-free mice colonized by IBD-associated-microbiota developed severe colitis compared to those that were colonized by healthy human microbiota. IBD patients are shown to have a reduction in gut firmicutes such as Faecalibacterium prausnitzii and Roseburia sp. These bacteria play an important anti-inflammatory role in reducing pro-inflammatory cytokines (IL-12, IFN-g)11. In total, the findings suggest that along with host genetics, diet, and antibiotic-induced dysbiosis, the gut microbiome is a key contributor to IBD.
Broad Spectrum Antibiotic Exposure Perturbs Gut MicrobiotaAs explained above, the gut microbiome plays a crucial role in animal health and shifts in the gut microbial community can have a detrimental impact on the host. Studies in mouse models have shown that antibiotic treatments greatly disrupt the native gut community, thereby facilitating the proliferation of pathogens. In fact, persistent infections following antibiotic treatment are a major medical issue. An interesting example of antibiotic perturbation of gut microbiome affecting host immunity involves Clostridium difficile. Secondary bile acids produced by gut microbiome protects the host against C. difficile infection4. Antibiotic administration perturbs the gut microbial communities and reduces their diversity, especially secondary bile acids-synthesizing dominant microbes, leading to reduced inhibition against C. difficile vegetative growth. This allows C. difficile outgrowth and colonization of the empty niches, resulting in impairment of intestinal ion absorption leading to gastrointestinal dysfunction3,12.
Figure 2: Broad-spectrum antibiotic-induced microbiome dysbiosis favors C. difficile growth
The gut microbiota plays an important role in growth and development and in the way the human body functions. The composition of gut microbiota is unique to everyone, just like fingerprints. It has been shown that the development of gut microbiota starts at birth. Scientists consider that by the age of 3, microbiota becomes stable and similar to that of adults, continuing its evolution at a steadier rate throughout life. Dysbiosis of gut microbiota can result due to aging, antibiotic intake, obesity, or diet. The human microbiome field of research is still relatively new but rapidly growing, showing several preliminary but promising studies on the modulatory role of microbiome in human wellness and disease